pd98059 calbiochem Search Results


mek1/2 inhibitor pd98059  (Cayman Chemical)
90
Cayman Chemical mek1/2 inhibitor pd98059
Mek1/2 Inhibitor Pd98059, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pd98059+calbiochem/pmc02527108-36-23-29?v=Cayman+Chemical
Average 90 stars, based on 1 article reviews
mek1/2 inhibitor pd98059 - by Bioz Stars, 2026-07
90/100 stars
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mek inhibitor pd98059  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology mek inhibitor pd98059
Mek Inhibitor Pd98059, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pd98059+calbiochem/pmc05058720-109-35-65?v=Santa+Cruz+Biotechnology
Average 94 stars, based on 1 article reviews
mek inhibitor pd98059 - by Bioz Stars, 2026-07
94/100 stars
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pd98059  (Bio-Techne corporation)
96
Bio-Techne corporation pd98059
Erk1/2 signaling is involved in LCA-induced miR21 through AP-1 transcription factor activation. ( A , B ) HCT116 cells were treated to <t>PD98059</t> 1 h prior to LCA treatment for 24 h. The cells were then harvested and checked for miR21 expression by Realtime-PCR. ( C ) HCT116 cells transfected with pGL3-miR21 promoter were treated with PD98059 and then 30 µM LCA for 24 h. The cells were then lysed and subjected for dual-luciferase assay for miR21 promoter activity. * p < 0.05 versus control; # p < 0.05 versus only LCA. The above data represent the means ± SD from triplicate measurements.
Pd98059, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pd98059+calbiochem/pmc08508661-138-0-8?v=Bio-Techne+corporation
Average 96 stars, based on 1 article reviews
pd98059 - by Bioz Stars, 2026-07
96/100 stars
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inhibitors  (Tocris)
96
Tocris inhibitors
Erk1/2 signaling is involved in LCA-induced miR21 through AP-1 transcription factor activation. ( A , B ) HCT116 cells were treated to <t>PD98059</t> 1 h prior to LCA treatment for 24 h. The cells were then harvested and checked for miR21 expression by Realtime-PCR. ( C ) HCT116 cells transfected with pGL3-miR21 promoter were treated with PD98059 and then 30 µM LCA for 24 h. The cells were then lysed and subjected for dual-luciferase assay for miR21 promoter activity. * p < 0.05 versus control; # p < 0.05 versus only LCA. The above data represent the means ± SD from triplicate measurements.
Inhibitors, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pd98059+calbiochem/10__1161_slash_circresaha__111__300142-470-9-16?v=Tocris
Average 96 stars, based on 1 article reviews
inhibitors - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier

Image Search Results


Erk1/2 signaling is involved in LCA-induced miR21 through AP-1 transcription factor activation. ( A , B ) HCT116 cells were treated to PD98059 1 h prior to LCA treatment for 24 h. The cells were then harvested and checked for miR21 expression by Realtime-PCR. ( C ) HCT116 cells transfected with pGL3-miR21 promoter were treated with PD98059 and then 30 µM LCA for 24 h. The cells were then lysed and subjected for dual-luciferase assay for miR21 promoter activity. * p < 0.05 versus control; # p < 0.05 versus only LCA. The above data represent the means ± SD from triplicate measurements.

Journal: International Journal of Molecular Sciences

Article Title: Lithocholic Acid Induces miR21, Promoting PTEN Inhibition via STAT3 and ERK-1/2 Signaling in Colorectal Cancer Cells

doi: 10.3390/ijms221910209

Figure Lengend Snippet: Erk1/2 signaling is involved in LCA-induced miR21 through AP-1 transcription factor activation. ( A , B ) HCT116 cells were treated to PD98059 1 h prior to LCA treatment for 24 h. The cells were then harvested and checked for miR21 expression by Realtime-PCR. ( C ) HCT116 cells transfected with pGL3-miR21 promoter were treated with PD98059 and then 30 µM LCA for 24 h. The cells were then lysed and subjected for dual-luciferase assay for miR21 promoter activity. * p < 0.05 versus control; # p < 0.05 versus only LCA. The above data represent the means ± SD from triplicate measurements.

Article Snippet: PD98059 (Calbiochem, San Diego, CA, USA) and SR11302 (Bio-Techne, Minneapolis, MN, USA) were dissolved in DMSO as 30 mM stocks, and stored at −80 °C.

Techniques: Activation Assay, Expressing, Transfection, Luciferase, Activity Assay

LCA inhibits PTEN expression via signals involved in the LCA-induced miR21 mechanism, Erk1/2-AP-1 and STAT3. HCT116 cells were treated with PD98059 at 10 µM–30 µM for 1 h prior to 30 µM LCA treatment for 24 h. The cells were then harvested and checked for PTEN expression by Realtime-PCR ( A ) and Western blot ( B ). HCT116 cells were treated to SR11302 at 10 µM–30 µM concentration for 1 h prior to LCA treatment and then subjected to check PTEN expression by Realtime-PCR ( C ) and Western blot ( D ). ( E ) HCT116 cells were transfected to 50 nM control siRNA (siCon) or siSTAT3, and then treated with 30 µM LCA for 24 h. The cells were further extracted for total RNA and checked for PTEN expression by Realtime-PCR. * p < 0.05 versus control; # p < 0.05 versus only LCA. The above data represent the means ± SD from triplicate measurements.

Journal: International Journal of Molecular Sciences

Article Title: Lithocholic Acid Induces miR21, Promoting PTEN Inhibition via STAT3 and ERK-1/2 Signaling in Colorectal Cancer Cells

doi: 10.3390/ijms221910209

Figure Lengend Snippet: LCA inhibits PTEN expression via signals involved in the LCA-induced miR21 mechanism, Erk1/2-AP-1 and STAT3. HCT116 cells were treated with PD98059 at 10 µM–30 µM for 1 h prior to 30 µM LCA treatment for 24 h. The cells were then harvested and checked for PTEN expression by Realtime-PCR ( A ) and Western blot ( B ). HCT116 cells were treated to SR11302 at 10 µM–30 µM concentration for 1 h prior to LCA treatment and then subjected to check PTEN expression by Realtime-PCR ( C ) and Western blot ( D ). ( E ) HCT116 cells were transfected to 50 nM control siRNA (siCon) or siSTAT3, and then treated with 30 µM LCA for 24 h. The cells were further extracted for total RNA and checked for PTEN expression by Realtime-PCR. * p < 0.05 versus control; # p < 0.05 versus only LCA. The above data represent the means ± SD from triplicate measurements.

Article Snippet: PD98059 (Calbiochem, San Diego, CA, USA) and SR11302 (Bio-Techne, Minneapolis, MN, USA) were dissolved in DMSO as 30 mM stocks, and stored at −80 °C.

Techniques: Expressing, Western Blot, Concentration Assay, Transfection